Topical formulations (Gel ) have been developed containing Chloroform Extracts of Azadirachta indica, chloroform extract of Ocimum sanctum and methanol Extracts of Curcuma longa and Aloe vera concentrate gel and almond oil. In vitro antibacterial activity was performed against Propionibacterium acnes (P. acnes), a causative organism for Acne vulgaris for the developed formulations using cup plate method. The measured zones of inhibitions of the formulations were compared with standard antibiotic (tetracycline), standard marketed topical herbal preparation for acne. Results of the investigation showed that formulation 1 has greater antibacterial activity (zones of inhibition >19.8 mm) than other formulations and which is comparable to that of standard marketed topical herbal preparation. When compared with chloroform extract of Ocimum sanctum (Zone of inhibition < 17 mm), chloroform extract of Azadiracta indica (Zone of inhibition < 32mm) and methanol Extracts of Curcuma longa (Zone of inhibition 22 mm) all formulations had shown better activity while the activity was found less on comparison with the standard antibiotic solution (zone of inhibition >34 mm). Invivo activity has done for the formulation 1. As physical observations were carried out on regular intervals at the rabbit ear and progressive fading of comedo was found on drug treated animals. No change was observed in control group, where as in test groups shown significant improvement on comedo reduction of rabbit pinnas was observed photographically. Test group showed a significant improvement compared to standard comedo.
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